Simple Isolation of Cordycepin from Cordyceps militaris by Dual-Normal Phase Column Chromatography and Its Potential for Making Kombucha Functional Products

Cordycepin (3?-deoxyadenosine) is a potent bioactive metabolite of the medicinal fungus Cordyceps militaris, which has been increasingly exploited to treat various chronic diseases in humans. However, current synthesis and purification procedures cordycepin are principally laborious complicated. This study provides simple protocol approach isolate purify from C. militaris by normal phase column chromatography at room temperature. Besides, this first investigate potential cordycepin-included extracts for making Kombucha functional products. By repeated chromatography, an amount 1.16 g isolated 2.8 kg fruiting bodies obtained efficiency 83.26% compared that estimated high-performance liquid (HPLC). The purity confirmed thin-layer (TLC), HPLC, proton nuclear magnetic resonance (1H NMR). In addition, kombucha-fermented fractions show biological activities terms antioxidant, anti-diabetes via ?-glucosidase inhibitory assay, cytotoxicity MTT assay on Meg-01 HL-60 cell lines. Further studies optimization extraction verification health benefits kombucha products should be conducted prior official mass production.